Biochemical analysis of the saliva of patients with cognitive and physical disabilities as a practical Biochemistry class strategy in the Dentistry course

Authors

  • Antonio Hernandes Chaves-Neto Professor (a) Assistente Doutor (a) da Disciplina de Bioquímica do Departamento de Ciências Básicas da Faculdade de Odontologia de Araçatuba da Universidade Estadual Paulista “Júlio de Mesquita Filho” - FOA/UNESP Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas, SBFIs, da Faculdade de Odontologia de Araçatuba da Universidade Estadual Paulista “Júlio de Mesquita Filho” - FOA/UNESP https://orcid.org/0000-0001-6481-5506
  • Maria Flávia Marques Ribeiro Professora Titular do Instituto de Ciências Fisiológicas da Universidade Federal do Rio Grande do Sul – UFRGS https://orcid.org/0000-0002-2265-7332
  • Ana Cláudia de Melo Stevanato Nakamune Universidade Estadual Paulista "Júlio de Mesquita Filho" Unesp, Faculdade de Odontologia de Araçatuba https://orcid.org/0000-0001-5098-8406

DOI:

https://doi.org/10.30979/rev.abeno.v20i2.1029

Keywords:

Learning. Teaching. Education, Dental. Biochemistry. Saliva.

Abstract

Given the importance of teaching biochemistry in dental surgeon training courses, the objective was to present a pedagogical strategy that enables the articulation of basic molecular concepts within the subject, allowing a better understanding of the complexity of oral and systemic diseases, through the biochemical analysis of saliva from patients with cognitive and physical disabilities at the Dental Assistance Center for Disabled  Persons (CAOE) of the School of Dentistry, Araçatuba - São Paulo State University (UNESP). For this purpose, groups of five students analyzed the saliva collected by a dental surgeon from CAOE. Immediately after collection, salivary flow, pH, and buffering capacity were determined. Subsequently, the saliva was centrifuged, fractionated, and stored at -20 °C until the time of biochemical analysis. During the practical classes, using commercial kits, the samples were tested for: total protein, α-amylase, alkaline phosphatase, uric acid, glucose, aspartate aminotransferase, calcium, and phosphorus. At the end of the school year, students presented a report contextualizing the results with the patient's oral and systemic health. Tutoring sessions with monitors and teachers helped in the interpretation of the anamnesis form and the correlations of oral and systemic clinical parameters. For incoming students, this was the first opportunity to integrate theoretical knowledge with clinical perspectives. It is concluded that the adopted strategy is viable and can benefit educators who seek alternatives that allow the integration of basic and clinical sciences for teaching Biochemistry in Dentistry.

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Published

10-11-2020

How to Cite

Chaves-Neto, A. H., Ribeiro, M. F. M., & Nakamune, A. C. de M. S. (2020). Biochemical analysis of the saliva of patients with cognitive and physical disabilities as a practical Biochemistry class strategy in the Dentistry course. Revista Da ABENO, 20(2), 137–146. https://doi.org/10.30979/rev.abeno.v20i2.1029

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